T4 DNA Ligase buffer

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T4 DNA Ligase Reaction Buffer | NEB10X reaction buffer supplied with T4 DNA Ligase. A 1X concentration assures optimal enzyme activity. twT4 DNA Ligase Buffer - Thermo Fisher ScientificT4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. twT4 DNA Ligase Buffer (10X) - Thermo Fisher ScientificThe Thermo Scientific 10X T4 DNA Ligase buffer is used with T4 DNA Ligase. The 10X buffer has been tested for absence of endo-, exodeoxyribonucleases, ... twT4 DNA ligase structure reveals a prototypical ATP-dependent ...2018年8月29日 · Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to ... concentrations with used dialysis buffer as measured by A257 (ϵ ...Comparative analysis of the end-joining activity of several DNA ligases2017年12月28日 · T4 DNA ligase reaction buffer (50 mM Tris-HCl pH 7.5 @ 25°C, 1 mM ATP and 10 mM MgCl2) as a 10x stock, NEBNext® Quick Ligation reaction ...The Inhibitory Effect of Non-Substrate and Substrate DNA on ... - PLOS2016年3月8日 · Samples were prepared with 5 μM deadenylylated T4 DNA ligase with and without 5 μM or 50 μM I-75-dsDNA in 1X ATP-free ligase reaction buffer in ...Engineered DNA ligases with improved activities in vitroThe DNA ligase from bacteriophage T4 is one of the most widely used enzymes ... and a ligase (2 µM), in 1× New England Biolabs T4 DNA Ligase Reaction Buffer ...Template‐independent ligation of single‐stranded DNA by T4 DNA ...2005年11月16日 · T4 DNA ligase is one of the workhorses of molecular biology and used in various ... Email; Facebook; Twitter; Linked In; Reddit; Wechat ...T4 DNA Ligase - Sigma-AldrichT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ ... III DNA fragment substrate in 1X T4 DNA Ligase reaction buffer. | T4 DNA Ligase Buffer Pack - Promega CorporationDesigned for use with T4 DNA Ligase (Cat.# M1801, M1804, M1794) to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the ...